Phenols have an antioxidant effect and can be used as natural antioxidants for curing ailments related with inflammation and oxidative stress. The study concludes that ethanol extract of Eisenia fetida coelomic fluid contains significant amount of phenols. Methanol is used as solvent for low molecular weight polyphenols. Phenols are more readily soluble in ethanol and safe for human consumption. Eisenia fetida ethanolic extract in the sample manifested high total phenolic content than methanolic extract. Extraction with 85% methanol on the other hand, yielded 189.1 mg GAE\L. It was analysed that the extraction of phenolic compound with 85% ethanol yielded high phenol content, 208.6 mg GAE\L Eisenia fetida. The study focused on total phenolic compound determination using two solvents in a single species of earthworm. The worms were cultured in lab until they became sexually mature. Antioxidant assay was performed to count the total phenolic content in coelomic fluid of Eisenia fetida.
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